ABSTRACT
Neuropeptides play an important role in the physiological functions of the human body. The physiological activities such as pain, sleep, mood, learning and memory are affected by neuropeptides. Neuropeptides mainly exist in the nerve tissue of the body, and a small amount of them are distributed in body fluid and organs. At present, analysis of large-scale identification of neuropeptides in whole brain tissue is still challenging. Therefore, high-throughput detection of these neuropeptides is greatly significant to understand the composition and function of neuropeptides. In this study, 1 830 endogenous peptides and 99 novel putative neuropeptides were identified by extraction of endogenous peptides from whole brain tissue of mice by liquid phase tandem mass spectrometry (LC-MS / MS). The identification of these endogenous peptides provides not only a reference value in the treatment and mechanism studies of diseases and the development of drugs, but also the basis for the study of a new neuropeptides and their functions.
ABSTRACT
Objective To analyze the chemical components of the liposoluble constituent of Holotrichia diomphalia lar-vae .Methods Essential liposoluble compounds were extracted from H .diomphalia through ethylacetate .GC-MS analysis was used to identify its main components .Results 33 peaks were separated and 33 compounds were identified from ethylacetate-ex-tracted H .diomphalia .The main compounds were pentadecylic acid ,Z-11-Hexadecenoic acid ,hexadecanoic acid ,heptade-canoic acid ,oleic acid and octadecanoic acid .Conclusion This work will contribute to the study of medicinal animals and con-firmation of active compounds in H .diomphalia .